The special is aimed at paying tribute to health workers and those on the front lines of efforts to cope with the pandemic, which has overwhelmed hospitals. It will also encourage viewers to donate to two charities: Feeding America and the First Responders Children's Foundation.
Musicians have been quick to move online as US states have issued shutdown and stay-at-home orders, with free performances filmed at home and streamed on social media from the likes of John Legend and Coldplay frontman Chris Martin.
Others have made individual contributions to hospitals and nonprofits as the coronavirus has spread across the United States.
They include folk singer James Taylor, who this week made a $1 million donation to Massachusetts General Hospital, and Rihanna, whose foundation gave $5 million to charities to supply food and protective equipment to healthcare workers.
Fox said the "IHeart Living Room Concert for America" will be broadcast on 0100 GMT on Monday.