Co-written with Canadian rapper Drake, the dance track appears to use the word "spaz" in the colloquial sense of temporarily losing control or acting erratically. But disability campaigners noted that the word is derived from "spastic".
According to the Centers for Disease Control and Prevention, spasticity is a movement disorder involving stiff muscles and awkward movement, suffered by 80 percent of people with cerebral palsy.
In June, US singer Lizzo re-recorded her song "Grrrls" to remove the same term following complaints that it was derogatory.
Australian disability campaigner Hannah Diviney said the inclusion of the word by Beyonce "feels like a slap in the face to me, the disabled community & the progress we tried to make with Lizzo."
"Guess I'll just keep telling the whole industry to 'do better' until ableist slurs disappear from music," she tweeted.
Beyonce's eagerly anticipated seventh solo studio album "Renaissance" was released Friday, drawing mainly positive reviews with its nods to disco and electronic dance.
Other collaborators on the album, which leaked online in the days prior to its official release, include Nile Rodgers, Skrillex, Nigerian singer Tems, Grace Jones, Pharrell and Beyonce's rap mogul husband Jay-Z.
In an Instagram post published soon after the album's release, Beyonce said creating the album "allowed me a place to dream and to find escape during a scary time for the world.”
"My intention was to create a safe place, a place without judgment. A place to be free of perfectionism and overthinking. A place to scream, release, feel freedom,” she wrote.