From cyberattacks on American entities and meddling in the last two US presidential elections, to human rights violations and aggression against Ukraine and other European countries, Washington's list of allegations against the Kremlin runs long.
Putin came to the summit arguing that Moscow is simply challenging US hegemony -- part of a bid to promote a so-called "multi-polar" world that has seen Russia draw close with the US's arguably even more powerful adversary China.
In a pre-summit interview with NBC News, he scoffed at allegations that he had anything to do with cyberattacks or the near-fatal poisoning of one of his last remaining domestic opponents, Alexei Navalny.
Biden, ending an intensive first foreign trip as president, arrived in Geneva after summits with NATO and the European Union in Brussels, and a G7 summit in Britain.
While in Brussels, he said he would detail his "red lines." "I'm not looking for conflict," he said, but "we will respond if Russia continues its harmful activities".
However, Biden, who had previously characterised Putin as a "killer", upgraded the Russian leader to "worthy adversary". And for all the rhetoric, the White House and Kremlin both say they are open to doing business in a limited way.
Officials point to the recent extension of the New START nuclear arms limitation treaty as an example of successful diplomacy.
Unlike in 2018, when Biden's predecessor Donald Trump met Putin in Helsinki, there was to be no joint press conference at the end of the summit.
The US side clearly wanted to avoid the optics of having Biden sharing that kind of platform with the Russian president.
In 2018, Trump caused a stir by saying, as Putin stood beside him, that he believed the Kremlin leader over his own intelligence services when it came to accusations of Russian interference in the 2016 US presidential election bringing Trump to power.