Data collected from the test will be used to finalize a date for Artemis-1 -- NASA had said May could be the first window, but later now seems likely.
It is called a "wet" dress rehearsal because super-cooled liquid hydrogen and liquid oxygen will be loaded into SLS from ground systems, just as they would be in a real launch.
The 322 feet (98 meters) tall rocket -- expected to be the most powerful in history at the time it is operational -- was rolled out to Launch Complex 39B at the Kennedy Space Center in Florida around two weeks ago.
The test begins at 5:00 pm Eastern Time (2100 GMT) with a "call to stations," as members of the launch control team arrive at their firing rooms and start a countdown of more than 45 hours.
With the SLS rocket and Orion crew capsule fixed on top powered on, teams will proceed to load 700,000 gallons (3.2 million liters) of propellant, and practice procedures such as pauses in countdown and other checks.
They won't actually ignite the rocket's RS-25 engines, which were tested previously. Instead they will halt the countdown about 10 seconds before liftoff, in order to simulate a "scrub," when launch is aborted due to technical or weather related issues.
The fuel will be drained, and a few days later SLS and Orion will be rolled back to the vehicle assembly building to carry out checks on how everything went.
Test milestones will be posted on NASA's blog for the Artemis mission, but the agency won't let the public listen to live internal audio, as it did in the past for Space Shuttle missions.
Whitmeyer explained this was because certain key information, including timing sequences, could assist other countries looking to develop long range missiles.
"We're really, really super sensitive to cryogenic launch vehicles that are of this size and capability, (and) are very analogous to ballistic type capabilities that our countries are very interested in," he said, but added that the agency could re-evaluate the position in future.