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Previous studies suggest that culturing such cells in simulated microgravity increases the efficiency of their production. But using live cell cultures in space presents some unique challenges. The MVP Cell-03 experiment, for example, must be conducted within a specific timeframe, when the cells are at just the right stage. Flight changes and crew availability could lead to delays that affect the research.

"Sometimes a flight is delayed and investigators have to prepare batches and batches of backup cells," said Chunhui Xu of Emory University School of Medicine in Atlanta, principal investigator for MVP Cell-03.

"Astronauts face an overwhelming amount of work the day investigations arrive, but these cells need fresh medium right away. We thought we had better work out this procedure ahead of time," Xu added.

The results, recently published in the journal Biomaterials, show that cryopreservation does not appear to affect the cells and even offers the added benefit of protecting cells from excess gravity experienced during launch.

The team also compared a new cell culture medium that does not require carbon dioxide with the current standard medium, which does, and found no difference between the two. Carbon dioxide adds weight and mass -- and cost -- to a space launch. The research team tested several modifications to culture media to improve cryopreservation procedures as well.

The cryopreserved heart cells flew to the space station in March 2020. Astronauts thawed and successfully cultured them, generating beating heart cells. Those were returned to Earth after 22 days of spaceflight.

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