The study found that the intercellular machinery of a cell assembled into a highly curved basket-like structure that eventually grew into a closed cage. Scientists had previously believed that structure began as a flat lattice.

Membrane curvature is important, Kural said, “It controls the formation of the pockets that carry substances into and out of a cell.”

The pockets capture substances around the cell, forming around the extracellular substances, before turning into vesicles -- small sacs one-one millionth the size of a red blood cell. Vesicles carry important things for a cell’s health -- proteins, for example -- into the cell. But they can also be hijacked by pathogens that can infect cells.

But the question of how those pockets formed from membranes that were previously believed to be flat had stymied researchers for nearly 40 years.

“It was a controversy in cellular studies. And we were able to use super-resolution fluorescence imaging to actually watch these pockets form within live cells, and so we could answer that question of how they are created,” Kural said.

“Simply put, in contrast to the previous studies, we made high-resolution movies of cells instead of taking snapshots,” Kural said.

“Our experiments revealed that protein scaffolds start deforming the underlying membrane as soon as they are recruited to the sites of vesicle formation,” Kural explained.

That contrasted with previous hypotheses that the protein scaffolded of a cell had to go through an energy-intensive reorganization in order for the membrane to curve, Kural said.

The way cells consume and expel vesicles plays a key role for living organisms. The process helps clear bad cholesterol from blood; it also transmits neural signals. The process is known to break down into several diseases, including cancer and Alzheimer’s disease.

“Understanding the origin and dynamics of membrane-bound vesicles is important -- they can be utilized for delivering drugs for medicinal purposes but, at the same time, hijacked by pathogens such as viruses to enter and infect cells. Our results matter, not only for our understanding of the fundamentals of life but also for developing better therapeutic strategies,” Kural said.

Emanuele Cocucci, an assistant professor in Ohio State’s College of Pharmacy, co-authored this study, along with researchers from UC Berkeley, UC Riverside, Iowa State University, Purdue University and the Chinese Academy of Sciences.

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