"We have identified a pathway that can suppress this cellular invasion, which could offer a new way to increase survival."
Despite decades of research, the prognosis for most patients with glioblastoma remains dismal, with median survival after diagnosis of just 15-18 months. Part of the challenge in treating this cancer is its invasive nature: Glioblastoma tumours invade surrounding healthy brain tissue, sending tentacle-like extensions out from the primary tumour that is impossible to remove with surgery alone and difficult to reach with chemotherapy.
Researchers have long considered the epidermal growth factor receptor (EGFR), a protein that sits on the surface of cells, as a driver of this cancer, Habib explained. In nearly half of glioblastoma patients, the gene that codes for EGFR is amplified, causing glioblastoma cells to produce far more molecular signals spurred by this protein and causing tumour cells to proliferate.
Consequently, Habib added, several clinical trials have focused on inhibiting EGFR - but each has failed to improve the prognosis for glioblastoma.
EGFR on glioblastoma cells can send these signals in two ways: either without prompting, a state known as constitutive signalling, or when stimulated with proteins called ligands. The differences between these two pathways have been considered inconsequential, Habib said.
Thus, glioblastoma patients with amplified EGFR have been grouped together in clinical trials.
In the new study, Habib and colleagues in the Habib lab and elsewhere showed that when cells with amplified EGFR were stimulated with ligands, this receptor appeared to act as a tumour suppressor, preventing invasion into healthy tissue both in laboratory and animal models.
Further experiments showed that a cytoskeletal protein called BIN3 appears to be responsible for inhibiting this invasion. When the researchers dosed animals with amplified EGFR glioblastoma tumours with an FDA-approved arthritis drug called tofacitinib that increased the amount of EGFR ligands and BIN3, tumours remained smaller and were less likely to invade healthy brain tissue. Additionally, these animals survived significantly longer than animals that didn't receive this drug.
Habib noted that tofacitinib could offer a new way to extend life for patients with both amplified EGFR and a relatively high level of EGFR ligands, a strategy he and his colleagues will explore in a clinical trial launching in September. For patients without high ligand numbers, he added, strategies previously explored to inhibit EGFR could potentially extend survival.
"These approaches could offer new tools in our arsenal to fight glioblastoma," Habib concluded.